So, I ran into a weird result last month while comparing IgM-heavy sera in a functional assay, and it got me thinking. From an evolutionary angle, how much do IgM’s complement-triggering habits skew what we think we’re seeing once secondary antibodies enter the mix?
6 Views
From my own bench time, IgM has a habit of quietly complicating things more than we expect. I once chased a signal for days before realizing the complement cascade was doing half the talking, not the target itself. When secondary antibodies come in, that early immune “overreaction” can blur interpretation. I remember reading up on the lgm full form just to sanity-check my assumptions, and it helped me frame IgM as more of a biological amplifier than a neat marker. That shift alone changed how I read my data.